Figure 2.

RPA analysis of MUC 2 mRNA expression in human MUC 2-expressing epithelial cell lines. Up-regulation of MUC 2 mRNA by P. aeruginosa culture supernatants occurred in both HM3 (human colon epithelial) and NCIH292 (human airway epithelial) cells. Cells were treated with P. aeruginosa culture supernatants (PA) or vehicle (Con) for 6 h before cell lysis and RNA extraction. The results are typical of four separate experiments for each cell line. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.