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. 1997 Feb 4;94(3):967–972. doi: 10.1073/pnas.94.3.967

Figure 3.

Figure 3

Up-regulation of MUC 2 transcriptional activity by P. aeruginosa. (A) A 2.8-kb DNA fragment of the 5′ flanking region of the human MUC 2 gene cloned into a luciferase reporter gene (p-2864luc) was transfected into NCIH292, HM3, and CFTE29O cells. Luciferase activity was then assessed in P. aeruginosa-treated and nontreated cells. Induction by P. aeruginosa was detected in all cell lines. (B) Comparison of the P. aeruginosa responsiveness of p-2864luc and p-73luc in NCIH292, HM3, and CFTE29O cells. Response elements reside between −2864 and −73 bp. (C) Comparison of the P. aeruginosa responsiveness of p-73luc, p-343luc, p-621luc, p-1308luc, and p-2864luc in HM3 cells. Transfected cells were treated with either P. aeruginosa culture supernatants or vehicle for 6 h before cell lysis. All transfections were carried out in triplicate. Values represent means ± SD (n = 5). Luciferase activity was normalized with respect to β-galactosidase activity.