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. 1997 Feb 4;94(3):967–972. doi: 10.1073/pnas.94.3.967

Figure 4.

Figure 4

Effect of P. aeruginosa LPS on MUC 2 transcriptional activity. HM3 cells were transfected with p-2864luc. (A) After 42 h, the cells were exposed to culture supernatants (CS) and LPS (5 μg/ml) purified from PAO1 and PA10 (serotype 10) (Sigma). (B) As in A, the cells were exposed to culture supernatants or purified LPS from PAO1 (wild-type) or PAO-pmm (algC) mutants. (C) The cells were exposed to LPS (5 μg/ml) (P. aeruginosa serotype 10) and E. coli lipid A (5 μg/ml) (Sigma). After 6 h, the cells were harvested for luciferase activity measurement. All transfections were carried out in triplicate. Values represent means ± SD (n = 4). Luciferase activity was normalized with respect to β-galactosidase.