Figure 1.

Characterization of chimeric transcriptional repressors. (A) Schematic illustration of repressor proteins. The KRAB, KRAB(DV), SNAG, Engrailed, BTB/POZ, and WT1 repression domains (RDs) were fused in frame with PAX3-HBD to generate the RD-PAX3-HBD fusion proteins. (B) Immunoprecipitation of transfected cell extracts with α-PAX3 IgG. Asterisks indicate the expressed proteins. MW, molecular weight markers. (C) 4-OHT-dependent repression of PAX3-luciferase reporter gene by the chimeric repressors. NIH3T3 cells were transfected with the indicated expression plasmids and the CD19-TK-LUC reporter plasmid. Post-transfection and 4-OHT treatment, cell lysates were assayed for luciferase and β-galactosidase activities. Fold repression represents the ratio of normalized luciferase activity of -/+OHT treated cells.