Figure 9E.

Molecular characteristics of the active and silent clones. (E) Sodium bisulfite-genomic sequencing. The bisulfite-modified genomic DNAs of the silent (cl 39-40) and active (cl 39-45) clones were PCR-amplified using UMS1 (sense) and UMA1 (antisense) primers. The PCR products were TA-cloned into pCR II vector (Invitrogen). A representative selection of clones from both cl 39-40 and cl 39-45 were sequenced and the results presented. Hanging ⋄, the positions of CpG residues; □, the unmethylated CpG; ▪, methylated CpG residues. Arrows pointing up mark the CpG residues that are preferentially methylated in the genomic clones of cl 39-40.