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Characterization of stable cell lines. (B,C) 4-OHT-dependent repression of chromatinized PAX3-luciferase reporter gene in stable cell lines. First (top) graphs represent the normalized luciferase activity (B) and fold repression (C) of five independent “CL” (luciferase reporter) clones. Second, third, and fourth graphs illustrate the normalized luciferase activity (B) and fold repression (C) in clones stably expressing the respective RD-PAX3-HBD proteins.
