Table 1.
Predicted SKN-1-binding sites upstream of C. elegans oxidative stress-resistance genes
| Enzymes | Gene or ORF | Locationa | Direction | Sequence |
|---|---|---|---|---|
| γ-Glutamyl-cysteine synthetase heavy chain (GCS(h)) | gcs-I | −121 | → | TTTATCAT |
| −316 | ← | ATGACTTA | ||
| −607 | ← | ATGACAAT | ||
| Glutathione synthetase | M176.2 | −137 | → | TTTGTCAT |
| −169 | ← | ATGACAAA | ||
| −243 | → | TTTATCAT | ||
| −378 | ← | ATGATTTT | ||
| NADH quinone oxidoreductase | F39B2.3 | −469 | → | GTTATCAT |
| −518 | ← | ATGACAAT | ||
| Glutathione S-transferase | R03D7.6 | −149 | ← | ATGACAAT |
| −282 | ← | ATGATTTT | ||
| −302 | ← | ATGACATT | ||
| −947 | ← | ATGATTTT | ||
| F35E8.8 | −94 | ← | ATGACAAT | |
| −240 | ← | ATGATAAT | ||
| F11G11.2 | −133 | ← | ATGACAAA | |
| −391 | → | CTTATCAT | ||
| K08F4.7 | −83 | ← | ATGACATT | |
| −157 | → | TTTGTCAT | ||
| Superoxide dismutase | sod-1 | −64 | → | ATAATCAT |
| sod-2 | −191 | → | TGTATCAT | |
| −363 | ← | ATGACAAT | ||
| −959 | → | AGAATCAT | ||
| −980 | → | AGAATCAT | ||
| sod-3 | −287 | → | TAAATCAT | |
| Catalase | ctl-1 | −880 | → | ATGATCAT |
| −978 | → | GTCATCAT | ||
| −997 | → | CTTATCAT |
The SKN-1-binding consensus is shown in Figure 1C.
a
The A within the translation initiation codon is designated as base 1.