Table 2.

Induction of GCS-1::GFP expression in the intestine by oxidative stress

	gcs-1::gfp				gcsδ2::gfp
Inducer	Low	Medium	High	N	Low	Medium	High	N
Control	90.8%	7.9%	1.3%	76	88.2%	10.3%	1.5%	68
Heat shock	10.5%	72.4%	17.1%	76	0.0%	14.0%	86.0%	86
paraquat	14.5%	67.1%	18.4%	76	21.3%	65.6%	13.1%	61

A representative set of experiments involving a mixed population of L2-young adult worms is shown, from which the percentages of animals in each expression category are listed. Induction of GCS-1::GFP expression was comparable among the different developmental stages analyzed. “Low” refers to animals similar to that in Figure 2A, in which intestinal GCS-1::GFP was apparent at modest levels anteriorly, or anteriorly and posteriorly. “High” indicates that GCS-1::GFP was present at high levels anteriorly and detectable throughout most of the intestine, as in Figure 2F. “Medium” refers to animals in which GCS-1::GFP was present at high levels anteriorly as in Figure 2F and possibly posteriorly, but was not detected in between. N indicates the number of animals analyzed from each transgenic strain.