Table 3.

Accumulation of SKN-1::GFP in intestinal nuclei in response to oxidative stress

Inducer	Low	Medium	High	N
Control	78.9%	14.5%	6.6%	76
Heat	5.6%	11.9%	82.5%	143
Paraquat	53.1%	43.8%	3.1%	64
M9, 5 min	74.7%	17.6%	7.7%	91
50 mM sodium azide, 5 min	0.8%	44.2%	55.0%	120

Mixed-stage L2-young adult transgenic worms were exposed to the indicated conditions. A representative set of experiments is shown, from which the percentages of animals in each category are listed. SKN-1 localization patterns did not differ significantly among the different developmental stages examined. M9 refers to the control incubation for the sodium azide experiment. In some animals treated with sodium azide, high levels of nuclear SKN-1 ::GFP appeared in <1 min (data not shown). “Low” refers to animals in which SKN-1 ::GFP was barely detectable in all intestinal nuclei, as shown in Figure 4F. “High” indicates that a very strong SKN-1 ::GFP signal was present in all intestinal nuclei, as in Figure 4G. “Medium” refers to animals in which nuclear SKN-1 ::GFP was present at high levels anteriorly or anteriorly and posteriorly, but was barely detectable midway through the intestine. N indicates the number of animals analyzed in each category.