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The SF2/ASF FF-DD mutant does not assemble with the Rev/RRE complex in vitro. Wild-type SF2/ASF and the FF-DD and RT mutants of SF2/ASF were cloned into recombinant baculovirus expression plasmids and expressed in Sf9 cells. REMSA was performed using these recombinant polyhistidine-tagged proteins after purification on nickel affinity columns. As indicated, REMSA was performed in the presence and absence of Rev and wild-type or mutant SF2/ASF and antibody to SF2/ASF.
