Figure 6.

BMAL1 is required for circadian oscillation of nuclear CLOCK in mouse liver. (A) Western blot analysis of nuclear and cytoplasm CLOCK expression in livers of control wild-type and Bmal1^-/- mice isolated at indicated circadian times. Note that longer exposure time was used for the nuclear fraction. (B) Quantitative analysis of CLOCK nuclear/cytoplasm distribution in livers of control mice. •, nuclear CLOCK; ○, cytoplasmic CLOCK. Mean data of three independent experiments are shown. Each value represents a percentage of the maximal level of CLOCK in the nucleus or the cytoplasm.