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Expression of VEGF mRNA in several AIDS–KS cells lines. (A) Total RNA (15 μg) from KSC10, KSC29, KSC13, KSC59, and KSY1 were electrophoresed, blotted, and hybridized to the human VEGF cDNA and β-actin probe. (B) Total RNA (15 μg) was isolated from KSC10, HUVEC, and AoSM and analyzed as in A. (C) Supernatants from equal numbers of cells from KSY1, KSC10, AoSM, HUVEC, and T1 were collected after 48 hr and analyzed for VEGF protein by ELISA.
