Figure 3.

Effect of VEGF antisense oligonucleotides on cell growth. (A) KS cells were treated with VEGF antisense AS-1, AS-2, AS-3, AS-4, AS-5, and scrambled oligonucleotides at concentrations of 5 μM. Cell proliferation was measured on day 5. (B) AIDS–KS cells were treated with VEGF AS-1, AS-3, and scrambled oligonucleotides at concentrations ranging from 1 to 10 μM; cell proliferation was measured on day 5. Several control cell lines were also treated with VEGF AS-1 oligonucleotides (C), VEGF AS-3 oligonucleotides (D), and VEGF scrambled oligonucleotides (E). Cell proliferation was measured on day 5, as described earlier. Data from all above experiments represent the mean ± SD of two separate experiments performed in quadruplicate. (F) Effect of rhVEGF on the growth of KS and HUVEC cells. Cells were seeded at 1 × 10⁴ cells per well in 24 plates and treated with rhVEGF (1 and 10 ng/ml) for 48 hr. Cell counts were performed, and the results represent the mean ± SD of an experiment performed in quadruplicate. (G) rhVEGF abrogates the effect of VEGF antisense on AIDS–KS cell growth. KS cells were seeded at a density of 1 × 10⁴ cells per well in 24-well plates. Cells were treated with 1 and 10 μM of AS-1 or AS-3 alone or with rhVEGF (10 ng/ml) on day 1 and day 2. Cell proliferation was measured after72 hr. The data represent the mean ± SD of two experiments performed in quadruplicate.