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. 2003 Aug 15;17(16):1957–1962. doi: 10.1101/gad.1099003

Figure 3.

Figure 3.

Association of Tel1with sites near the HO-induced DSB. (A) The Tel1association with DSBs in SAE2 and sae2Δ cells. Cells expressing Tel1-HA (KSC1785 and KSC1786) were transformed with the YCpA-GAL-HO plasmid. Transformed cells were grown in sucrose and treated with nocodazole. After arrest at G2/M, the culture was incubated with galactose to induce HO expression, while part of the culture was maintained in sucrose to repress HO expression. Aliquots of cells were collected at the indicated times and subjected to chromatin immunoprecipitation. PCR was done with the primers for the ADH4 locus shown schematically in Figure 2A and for the control SMC2 locus. PCR products from the respective input extracts are shown in parallel. (B) Effect of the sae2Δ mutation on Tel1 association with DSBs in mec1Δ mutants. Cells expressing Tel1-HA (KSC1873, KSC1874 and KSC1875) were analyzed as in A. (C) Effect of xrs2Δ and xrs2-11 mutations on Tel1association with the DSBs. Cells expressing Tel1-HA (KSC1785, KSC1881 and KSC1882) were analyzed as in the top two panels of B. Immunoprecipitates were also subjected to immunoblotting analysis with anti-HA antibodies (bottom).