Table 1.
Intracellular mobility of Stat1 variant proteins
| Cytosol unstimulated (sec) | Nucleus unstimulated (sec) | Nucleus IFN (sec) | Nucleus IFN + vanadate (sec) | |
|---|---|---|---|---|
| wt | 45.5 ± 8.1 (n = 9) | 14.7 ± 3.3 (n = 9) | 18.6 ± 3.2 (n = 12) | 46.4 ± 11.2 (n = 9)* * |
| dnaplus | 41.8 ± 14.7 (n = 7) | 14.3 ± 3.7 (n = 4) | 206.7 ± 30.1 (n = 8)* | 233.7 ± 46.6 (n = 7)* |
| dnaminus | 37.8 ± 7.4 (n = 4) | 20.8 ± 6.5 (n = 4)* | 20.2 ± 4.1 (n = 4) | 36.6 ± 14.9 (n = 4)* |
| dnaoff | 39.6 ± 6.3 (n = 4) | 15.3 ± 7.8 (n = 5) | 22.8 ± 5.8 (n = 4) | 39.5 ± 5.4 (n = 4)* * |
Fluorescence recovery times of Stat1-GFP as determined by FRAP. They are expressed as mean ± SEM. Unpaired Student's t tests were performed to compare the intracellular mobility between Stat1 wt (row 1) and the mutants (rows 2-4). This was done for unstimulated cells (columns 1 and 2) and for cells treated with IFNγ (columns 3 and 4). Statistically significant differences (p < 0.05) with Stat1 wt are indicated by *. In addition, the recovery time recorded for each variant in the presence of IFNγ (column 3) was compared with the respective recovery time after the addition of vanadate/H2O2 (column 4). Here for each variant, significant differences (p < 0.05) are indicated by * *.