Table 1.
5′ RACE determination of transcript starts for WS PAI1 and Col PAI1 and PAI2
| Transcript | First upstream exona | Second upstream exona | First PAI exona |
|---|---|---|---|
| WS PAI1 | -868 to -803 | -386 to -361 | -96 to +10 |
| WS PAI1 | -868 to -803 | -386 to -361 | -96 to +10 |
| WS PAI1 | -855 to -803 | -386 to -361 | -96 to +10 |
| WS PAI1 | -851 to -803 | -386 to -361 | -96 to +10 |
| WS PAI1 | -868 to -803 | NA | -96 to +10 |
| WS PAI1 | -863 to -803 | NA | -96 to +10 |
| WS PAI1 | -860 to -803 | NA | -96 to +10 |
| WS PAI1 | -860 to -803 | NA | -96 to +10 |
| Col PAI1 | -847 to -777 | -386 to -361 | -96 to +10 |
| Col PAI1 | -845 to -777 | -386 to -361 | -96 to +10 |
| Col PAI1 | -845 to -777 | -383 to -361 | -96 to +10 |
| Col PAI1 | -842 to -777 | -386 to -361 | -96 to +10 |
| Col PAI1 | -834 to -777 | -386 to -361 | -96 to +10 |
| Col PAI1 | -834 to -777 | NA | -96 to +10 |
| Col PAI1 | NA | NA | -117 to +10 |
| Col PAI1 | NA | NA | -93 to +10 |
| Col PAI2 | NA | NA | -133 to +10 |
| Col PAI2 | NA | NA | -122 to +10 |
| Col PAI2 | NA | NA | -117 to +10 |
| Col PAI2 | NA | NA | -117 to +10 |
| Col PAI2 | NA | NA | -117 to +10 |
| Col PAI2 | NA | NA | -114 to +10 |
| Col PAI2 | NA | NA | -108 to +10 |
| Col PAI2 | NA | NA | -99 to +10 |
a
Numbering is relative to the first base of the PAI ATG translational start codon as +1 on genomic sequences. The WS genomic sequence (GenBank accession no. U34757) carries an extra 26 bp in the first intron region upstream of PAI1 relative to the Col genomic sequence (GenBank accession no. AF130878). The PAI sequence identity/methylation boundary ends at -355 in the WS genomic sequence.