Table 1.
Strain | Relevant phenotype
|
Selection* | No. of experiments | |||||
---|---|---|---|---|---|---|---|---|
Susceptibility to
|
Immunoblot presence of S layer, kDa | Survival, ×10−4
|
||||||
Chloramphenicol | Kanamycin | Serum | Mean | Range | ||||
23D:AC100 | R | S | S | — | Serum | 7 | 1.0 | 0.2–3.1 |
23D:AC200 | S | S | R | 127 | Chloramphenicol | 6 | 1.2 | 0.1–2.5 |
23D:ACA2K101 | S | R | S | — | Chloramphenicol | 12 | 0.7 | 0.2–2.5 |
23D:ACA2K101 | S | R | S | — | Serum | 8 | 1.2 | 0.2–1.4 |
23D:ACA2K200 | R | S | S | — | Kanamycin | 3 | 0.8 | 0.4–1.1 |
23D:ACA2K400 | S | S | R | 97 | Kanamycin | 6 | 1.0 | 0.8–1.4 |
23D:ACA2K400 | S | S | R | 97 | Chloramphenicol | 6 | 1.2 | 0.6–2.2 |
23D:ACA2K450 | S | S | R | 127 | Kanamycin | 5 | 2.6 | 1.2–4.6 |
23D:ACA2K450 | S | S | R | 127 | Chloramphenicol | 6 | 2.5 | 1.0–4.6 |
S, Sensitive; R, resistant.
Selection was performed by plating cells to media supplemented with 15 μg/ml chloramphenicol or 30 μg/ml kanamycin, or incubating cells in 10% NHS for 60 min at 37°C.