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. 1997 Feb 4;94(3):985–990. doi: 10.1073/pnas.94.3.985

Table 1.

C. fetus survival after serum or antibiotic selection

Strain Relevant phenotype
Selection* No. of experiments
Susceptibility to
Immunoblot presence of S layer, kDa Survival, ×10−4
Chloramphenicol Kanamycin Serum Mean Range
23D:AC100 R S S Serum 7 1.0 0.2–3.1
23D:AC200 S S R 127 Chloramphenicol 6 1.2 0.1–2.5
23D:ACA2K101 S R S Chloramphenicol 12 0.7 0.2–2.5
23D:ACA2K101 S R S Serum 8 1.2 0.2–1.4
23D:ACA2K200 R S S Kanamycin 3 0.8 0.4–1.1
23D:ACA2K400 S S R 97 Kanamycin 6 1.0 0.8–1.4
23D:ACA2K400 S S R 97 Chloramphenicol 6 1.2 0.6–2.2
23D:ACA2K450 S S R 127 Kanamycin 5 2.6 1.2–4.6
23D:ACA2K450 S S R 127 Chloramphenicol 6 2.5 1.0–4.6

S, Sensitive; R, resistant. 

*

Selection was performed by plating cells to media supplemented with 15 μg/ml chloramphenicol or 30 μg/ml kanamycin, or incubating cells in 10% NHS for 60 min at 37°C.