Figure 1.

Enhancement of BK current by NT-3 in cortical neuron. (A) Effect of NT-3 on the voltage-activated whole-cell current. The pulse protocol used in all experiments was a holding potential of 0 mV, voltage steps to 100 mV for 100 msec, and then a return to 0 mV for 5 sec. The current traces were obtained in buffer solution (Control) 20 min after exposure to NT-3 (50 ng/ml) and 2 min after administration of the BK channel blocker paxilline (1 μM). The leak current was small and constant during the experiment and has been subtracted. (B) Current vs. voltage relationship (I–V curve) under control conditions as well as 6 and 18 min after exposure to 50 ng/ml NT-3.