Figure 5.
BK channel activation by NT-3 was mediated by protein kinases, involved PLC, and was Ca2+-dependent. (A) Addition of 200 nM K252a (K) inhibited the stimulation of BK current by 50 ng/ml NT-3 (N) (four out of four cells). (B) Pretreatment with 1 μM U73122 (U) blocked the effect of NT-3 on BK current. (C) Addition of 1 μM U73343 (V) was devoid of effect (two out of two cells). (D) In the absence of extracellular Ca2+ (No Ca) the BK current decreased and NT-3 had no stimulatory effect (12 out of 12 cells). (T = 3 mM TEA.)