Figure 2.

Subcellular localization of GIRK1 IR in dendrites and dendritic spines in the CA1 region of hippocampus. (A) A dendritic spine in str. radiatum contains GIRK1–immunogold labeling (arrows). The labeled spine forms an asymmetric synapse (curved arrow) with an unlabeled axon terminal (uT). (B) A dendritic spine head with GIRK1–immunoperoxidase labeling (arrows) forms a synapse (curved arrow) with an unlabeled axon terminal (uT) in superficial str. radiatum. An adjacent unlabeled spine (uS) synapses with the same unlabeled terminal. (C) A dendrite labeled with GIRK1–immunoperoxidase in str. lacunosum-moleculare. The labeled dendrite forms a synapse (curved arrow), which is partially obscured by peroxidase reaction product, with an unlabeled terminal (uT). (Bar = 0.5 μm.)