Analysis of RT-PCR products from UMR-106.01 cells and primary cultures of HBMC indicating the presence or absence of expression of α-, β-, or γ-ENaC mRNA. (A) Lanes: 1, DNA molecular weight markers; 2, expected 0.6-kb α-rENaC product from UMR-106.01 cell RNA; 3, expected 0.6-kb α-hENaC product from HBMC RNA. (B) Lanes: 1, DNA molecular weight markers; 2, expected 1.1-kb β-rENaC product from UMR-106.01 cell RNA; 3, no RT control for β-rENaC product from UMR-106.01 cell RNA; 4, negative result for the expected 1.1-kb γ-rENaC product from UMR-106.01; 5, no RT control for γ-rENaC product from UMR-106.01 cell RNA.