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Increase in channel activity in cell-attached patches of LM/α-rENaC transfected cells plotted as a function of negative pipet pressure. Data were calculated by comparing NPo before and after a 2-sec square wave pulse of regulated negative pressure applied to the patch pipet through a computer-controlled solenoid valve. Patches (n = 15) were hyperpolarized by 50 mV relative to the cell. All increases of NPo were significantly different from control (P < 0.05).
