Table 1. Oligonucleotide primers used for kRT-PCR.

Gene	Primers	Primer sequence (5′ to 3′)	Gene Location	Base pair size
pmpA	pmpA-3 a	TGCTAGGGAAGATGTTGCAAATAG	1434–1457	51
	pmpA-4 a	TGAACGGGTTGGTTAAAAATCG	1484–1463
pmpB	pmpB-5 a	CGACTATCAGCAAAAACACTGCTAA	2120–2144	102
	pmpB-6 a	TAGCGGAGTTCTCAGAGATATTCAGTT	2221–2195
pmpC	pmpC-11 a	TTAGTGCTCCCTACAGACTCATCAA	4150–4174	56
	pmpC-12 a	CCCGTCAGTACTATTTTCTGAGCTT	4205–4181
pmpD	pmpD-3 a	GCGTGTCGCTCTGGAAAATAAT	4455–4476	51
	pmpD-4 a	ACTGTGCTGAAGTAAGAACTCCATTC	4505–4480
pmpE	pmpE-1 a	CATATGCGCTCTTCCGGATAC	2140–2160	51
	pmpE-2 a	GTGTGTCTGCCCTGCTATCATC	2190–2169
pmpF	pmpF-5 a	TCCTATGTTTGATCGCATTGCT	2520–2541	69
	pmpF-6 a	CTCCGCATGTTATGTGTTCCA	2588–2566
pmpG	pmpG-1 a , b	TGGGTTTCTGGAGTTTCGAATT	2221–2242	51
	pmpG-2 a , b	ACCTAAAGCATCGCGGTCAT	2271–2252
	pmpG-3 a	TGTGGCCCTGTACAATTCTTAGG	1165–1187	52
	pmpG-4 a	AAATCGCTCCACCATCATTAGC	1216–1195
pmpH	pmpH-15 a	TGCATACGCAGTATTTTAATGACAAA	2486–2511	61
	pmpH-16 a	TGCCAATGACATTTCGAATGAT	2546–2525
pmpI	pmpI-1 a	GGAGAAGTGTGCGCATCGAT	2176–2195	51
	pmpI-2 a	GAACAGTCCGGAACCATTGG	2226–2207
ompA	OmpA-9 c	TGCCGCTTTGAGTTCTGCTT	33–52	75
	OmpA-10 c	GTCGATCATAAGGCTTGGTTCAG	108–86
16SrRNA	16SRNA-9 d	GCGAAGGCGCTTTTCTAATTTAT	734–756	76
	16SRNA-10 d	CCAGGGTATCTAATCCTGTTTGCT	809–786

^aPrimers designed based on each pmp sequence of reference strains E/Bour and L₂/434 [12].

^bPrimers designed only for strain L₂.

^cPrimers designed based on the ompA sequence of reference strains E/Bour and L₂/434 (GenBank Accession No. X52557 and M14738, respectively).

^dPrimers designed based on the 16SrRNA sequence of reference strains E/Bour and L₂/434 (GenBank Accession No. D85722 and U68443, respectively).