Table 2. Number of crosses showing amplification of maternal and paternal haplotypes.* .
1 Day* | 3 Weeks | 6 Weeks | 9 Weeks | 16 Months | Total | Total | ||||||
mat | both | mat | both | mat | both | mat | both | mat | both | mat | both | |
cassini M×decim F | 10 | 1 | 2 | 12 | 5 | 8 | 0 | 4 | 5 | 10 | 22 | 35 |
decula M×decim F | 8 | 0 | 9 | 3 | 6 | 3 | - | - | - | - | 23 | 6 |
decim M×cassini F | 3 | 0 | 0 | 1 | - | - | 4 | 1 | - | - | 7 | 2 |
decim M×decula F | - | - | 0 | 1 | 0 | 1 | - | - | - | - | 0 | 2 |
decim×decim | - | - | - | - | - | - | 5 | U | 13 | U | 18 | U |
cassini×cassini | - | - | - | - | - | - | 3 | U | - | - | 3 | U |
total | 21 | 1 | 11 | 17 | 11 | 12 | 12 | 5 | 18 | 10 | 73 | 45 |
“mat” tested positive for maternal mtDNA only; “both” tested positive for both maternal and paternal mtDNA; “U” paternal inheritance cannot be determined because the paternal genome is identical to the maternal; “-” no eggs or nymphs were collected for this cross at this time period or samples that were collected failed to extract/amplify (only one pooled sample of 6-week old eggs failed to extract–a -decula M×-decim F; only two extractions failed to amplify and both of these were -decim M×-decim F 16-month old nymphs). Fewer offspring from crosses involving -decim males and heterospecific females were sampled because -decula and -cassini females most often rejected -decim males.