Table 2.
Binding activity of 125I-labeled GE with solubilized fraction from soybean root and tobacco suspension cells
| Samples | Transformation vector* (stable clone) | Binding sites, fmol/mg |
|---|---|---|
| Soybean root (soil) | − | 26.6 ± 2.8 |
| Soybean root (water) | − | 107 ± 11.6 |
| Tobacco suspension cells | − | ND |
| Tobacco suspension cells | Vector (transient) | ND |
| Tobacco suspension cells | GEBP (transient) | 90.5 ± 7.7 |
| Tobacco suspension cells | Vector (C2-1) | ND |
| Tobacco suspension cells | Vector (C2-4) | ND |
| Tobacco suspension cells | GEBP (I1) | 150 ± 17.2 |
| Tobacco suspension cells | GEBP (I6) | 196 ± 17.8 |
ND, Not detected.
*
No vector (−), control vector (Vector), or GEBP-expressed vector (GEBP) was used to transform tobacco cells.