Figure 3.
RNAi inactivation of ftt-2 results in the up-regulation of daf-16-dependent mRNA expression of sod-3 (A), C24B9.9 (B) and F53C3.12 (C). N2 worms or daf-16 (mgDf47) null mutant worms were exposed to the indicated RNAi starting as L1 at 16°C. Total RNA was extracted from young adult RNAi worms for real-time-PCR analysis. The y-axis indicates the RNA levels normalized to the RNA expression levels of the internal control act-1. The relative RNA levels for N2 worms treated with the L4440 control RNAi is set as 1. The average of four independent experiments is shown and the error bars represent SEM.