Figure 3. trc function is not required for self-avoidance in class I da neurons and prevention of dendritic bundling in class IV da neurons.

(A–D) MARCM clones of class I ddaE neurons mutant for trc¹ (A) and Dscam¹⁸ (B) are shown and dendritic bundling (C) and crossing (D) was quantified for both genotypes. Dendritic bundling events were defined as the physical contact between two or more branches over a distance of more than 5 μm. Scale bar, 30 μm.

(E–H) MARCM clones of class IV ddaC neurons mutant for trc¹ (E) and Dscam¹⁸ (F) are shown and dendritic bundling (G) and crossing (H) was quantified for both genotypes. While both trc and Dscam loss of function caused crossing defects, bundling of dendrites was only observed in the Dscam mutant. Scale bar, 30 μm. **, P<0.01, student’s t-test.