Table 1.
Experimental Design.
| Experiment | HIV Status | Treatment | Measurements | Duration of Experiment |
|---|---|---|---|---|
| 1 | HIV+ | HAART (n=8) | Morris Maze MAP-2 Percent Infected Cells Gliosis | 18 days |
| Vehicle (n=8) | ||||
| HIV− | HAART (n=8) | |||
| Vehicle (n=8) | ||||
| 2 | HIV+ | HAART (n=6) | TNF-α mRNA | 14 days |
| Vehicle (n=7) | ||||
| HIV− | HAART (n=5) | |||
| Vehicle (n=7) | ||||
| 3 | HIV+ | No Treatment (n=3) | Neuronal Apoptosis | 18 days |
| HIV− | No Treatment (n=3) |
*
Number of days after IC inoculation of cells that mice were sacrificed for pathological analyses.
#
Apoptosis was analyzed 3 ways (see Methods) including Apoptag staining of brain sections, caspase -3 detection by immunofluorescence in brain sections, and Western blots for caspase-3 expression in brain.