Figure 2.
Southern blot analysis of plants regenerated from hygromycin-resistant calli. DNA (1–5 μg) was digested with appropriate restriction enzymes, separated on 0.7% agarose gel and hybridized to a DIG-labeled gus (A) or cryIA(b)(B) probe. (A) DNA digested with BamHI. Lanes: 2–9, plants transformed with pKUB; 10–11, plants transformed with pKUC; 12–13, plants transformed with pKSB; and 14–17, plants transformed with pKBB. (B) DNA digested with HindIII. Lanes: 1, pKUB digested with HindIII; 2, untransformed rice plant; 3–7, plants (cv. 93VA) transformed with pKUC; 8–13, plants (cv. Zhong8215) transformed with pKUB; 14–16, plants (cv. T8340) transformed with pKSB; and 17–19, plants (cv. Kaybonnet) transformed with pKBB.