Figure 4.

Redox state analysis of DsbA and DsbC in fermentations. Samples were taken at the hours indicated and processed as described in Experimental Procedures. When the dsb gene was present on the plasmid, half the volume of whole cell extract was loaded relative to wild-type conditions prior to addition of l-arabinose (7 h time point only). After arabinose addition, a 15-fold dilution of the extract was loaded (remaining time points). Standards were generated in vitro and correspond to approximately 3 ng of each. IGF-I expression starts at approximately 14 h.