. Author manuscript; available in PMC: 2007 Sep 4.

Published in final edited form as: Anal Biochem. 2006 May 11;355(1):71–80. doi: 10.1016/j.ab.2006.04.045

Table 1.

Ranges for apparent limit of solubility, autohydrolysis rates, and k_cat/K_m ratios of Xuorescent substrates for sEH in aqueous solution

Substrate		Apparent limit of solubility (μM)	Autohydrolysis rate (pmol min⁻¹)	HsEH k_cat/K_m (s⁻¹ μM⁻¹)
Structure	#^a
	2	25 < LS < 50	5.0 ± 0.3	0.093 ± 0.007
	3	50 < LS < 75	1.6 ± 0.4	0.115 ± 0.004
	4	25 < LS < 50	1.8 ± 0.4	0.027 ± 0.001
	5	25 < LS < 50	2.1 ± 0.4	0.194 ± 0.009
	6	25 < LS < 50	2.0 ± 0.4	0.00119 ± 0.00002
	7	15 < LS < 25	3.6 ± 0.7	0.556 ± 0.073
	8	25 < LS < 50	6.4 ± 0.3	0.554 ± 0.018
	9	15 < LS < 25	2.3 ± 0.1	0.387 ± 0.084

Note. Apparent limit of solubility (LS) was determined by photometric turbidity analysis at 800 nm. Autohydrolysis rates of 10-μM substrate solutions are given as averages ± standard deviations (n = 30). SpeciWcity constants k_cat/K_m values are provided as means ± standard deviations (n = 3).

Substrate numbers (#) correspond to those in Jones and coworkers [24].