Table 2.

Results of the checkerboard assay for the optimization of HsEH and PHOME concentrations

[PHOME] (μM)	[HsEH] (nM)
	26.1	13.1	6.5	3.3	2.6	1.6	1.3	0.8	0.7	0.4	0.3	0
50	0.838	0.991	0.997	0.998	0.998	0.998	0.999	0.998	0.998	0.998	0.996	0.976
	12.4	10.8	7.3	4.3	3.8	2.6	2.3	1.8	1.6	1.4	1.3	1.0
	86	74	48	26	22	14	11	8	7	5	4	2
45	0.818	0.990	0.997	0.997	0.997	0.997	0.997	0.997	0.997	0.995	0.995	0.928
	22.3	20.2	12.9	7.2	5.7	4.0	3.1	2.4	2.0	1.7	1.4	1.0
	95	86	54	28	21	14	10	7	5	4	3	0.5
40	0.791	0.988	0.997	0.998	0.998	0.997	0.998	0.998	0.997	0.997	0.996	0.947
	23.6	22.1	15.3	8.4	6.8	4.6	3.4	2.8	2.3	1.8	1.7	1.0
	100	94	64	33	26	17	11	8	/6	4	3	1
35	0.730	0.952	0.997	0.998	0.997	0.997	0.997	0.996	0.997	0.996	0.997	0.953
	23.0	21.7	16.5	9.3	7.5	5.0	3.8	2.9	2.5	2.0	1.6	1.0
	100	97	73	40	31	19	14	10	8	6	4	1
30	0.671	0.932	0.997	0.998	0.998	0.997	0.997	0.998	0.998	0.996	0.997	0.887
	24.0	23.4	19.9	11.2	8.8	5.8	4.6	3.4	2.8	2.2	1.9	1.0
	100	62	53	29	22	4	10	7	5	4	3	1
25	0.579	0.841	0.985	0.998	0.998	0.998	0.998	0.998	0.997	0.997	0.996	0.889
	20.9	20.3	19.0	11.7	9.7	6.0	4.8	3.5	2.9	2.2	1.8	1.0
	100	100	97	58	47	28	21	14	11	7	6	1
20	0.521	0.761	0.937	0.815	0.998	0.998	0.998	0.998	0.998	0.997	0.997	0.963
	20.4	18.3	18.3	7.3	11.0	7.4	5.4	4.2	3.2	2.5	2.1	1.0
	100	100	100	41	60	39	28	20	14	10	8	1
15	0.474	0.701	0.874	0.998	0.998	0.998	0.998	0.998	0.998	0.998	0.998	0.958
	19.1	18.6	17.3	14.8	11.9	7.6	5.7	4.2	3.4	2.5	2.3	1.0
	100	100	100	100	80	50	36	25	19	13	11	2

Note. The three numbers given for each combination of concentrations represent, from top to bottom, (i) the squared linear correlation coefficient (r²) for 60 min of incubation time, (ii) the signal/background ratio after 60 min, and (iii) the percentage of substrate that was turned over after 60 min.