. Author manuscript; available in PMC: 2007 Sep 4.

Published in final edited form as: Anal Biochem. 2006 May 11;355(1):71–80. doi: 10.1016/j.ab.2006.04.045

Table 4.

Precision and accuracy data and Z′ values acquired from the fluorescent endpoint system validation

	Nominal inhibition (%)	Day 1		Day 2		Day 3		Interday precision^b (CV %)
	Nominal inhibition (%)	Mean accuracy^a (%)	Precision^a (CV %)	Mean accuracy^a (%)	Precision^a (CV %)	Mean accuracy^a (%)	Precision^a (CV %)	Interday precision^b (CV %)
Blank	—		3.4		2.2		3.4	4.6
Control	—		4.6		7.3		6.7	6.2
Inhibitors
CEU	5	95.2	5.2	71.3	3.9	59.0	3.5	5.0
CHU	35	97.9	3.7	92.4	2.0	70.0	6.2	5.7
CDU	55	99.9	2.5	95.0	3.9	88.6	2.6	5.7
AUDA	65	98.9	3.5	98.9	2.2	87.1	2.5	6.0
DCU	95	98.6	2.8	99.8	3.0	98.3	2.9	4.9
Z′		0.8^c		0.8^c		0.7^c		0.7^d

Note. Mean accuracy was determined by calculating the ratio of the determined inhibition percentage divided by the nominal value and multiplying it by 100%. Precision or coefficient of variation (CV) values represent standard deviations of fluorescent signal divided by the mean fluorescent signal of maximum enzyme activity and multiplied by 100%.

Intraday (n = 16).

Intraday (n = 48).

Intraday average (n = 4).

Interday average (n = 12).