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. 2007 Sep 6;117(10):2778–2790. doi: 10.1172/JCI30248

Figure 6. Ghrelin increases lymphoid progenitor populations in aging mice.

Figure 6

(A) Ghrelin increased lymphoid progenitor populations in 14-month-old mice without altering the peripheral CD4/CD8 ratios. (B) The ETPs were identified as LinlowcKit+CD25–. The lineage cocktail included antibodies against CD3ε, CD8α, TCRαβ, γδTCR, NK1.1, TER119, CD11b, CD11c, CD19, CD127, and B220 but not CD4. For ETP analysis, 106 thymocytes from 14-month-old BALB/c mice were stained for Lin–PE, CD25 PerCP, and c-kit FITC, and 700,000 total events were acquired. Linlow cells were analyzed for c-kit and CD25 expression. (C) Ghrelin infusion in 14-month-old mice led to a 2-fold increase in ETPs in thymus (P < 0.05) with no significant change in DN2 (LinlowcKit+CD25+), DN3 (LinlowcKit–CD25+), or DN4 populations. (D) Bone marrow cells were analyzed for LSK populations. (E) Ghrelin infusion significantly increased (P < 0.05) the BM LSK cells in 14-month-old animals. Data is represented as mean ± SEM of 2-month-old and 14-month-old mice (n = 6 per group).