Figure 2. DIO and body composition in wild-type OPN^+/+ and OPN^–/– mice.

(A) Wild-type OPN^+/+ (black symbols, n = 12) and OPN^–/– (white symbols, n = 12) mice were fed a LFD (circles) or HFD (squares). Weight gain was followed for 25 weeks and data expressed as mean ± SEM. Body composition (fat mass, lean mass, and body mass) before (B) and after (C) feeding a HFD (n = 7–8/group) was analyzed in OPN^+/+ (black bars) and OPN^–/– (white bars) mice by quantitative NMR. Data are presented as mean ± SEM. ^#P < 0.05, compared with OPN^+/+ mice.