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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Mol Biochem Parasitol. 2006 Dec 22;152(1):80–89. doi: 10.1016/j.molbiopara.2006.12.001

Fig. 3.

Fig. 3

gPP2A‐C mRNA and protein are upregulated in cysts and during excystation stage 1.

Upper panel: SAGE analyses show steady state mRNA levels in vegetative trophozoites (T), total cultures encysting for 4, 21 or 42 h, cysts (C), stage 1 (S1) and stage 2 (S2) excysting cells and 30 and 60 min after excystation.

Lower panel: Western blot analysis of total cell protein from the same stages in Giardia differentiation. The highest amount of gPP2A‐C protein was detected in cysts and Stage 1 of excystation relative to the PDI‐2 protein loading control shown at the bottom of the figure (PDI‐2).