Figure 1. Western Blotting PrP^Sc deposition in scrapie-affected Sarda sheep.

(A) Detection of PrP^Sc by conventional Western blot in the cortical and medullar part of kidneys. Brain and kidney homogenates were treated with (+) or without (−) proteinase K (PK), amounts of kidney and brain represented in each line are 1mg and 1g respectively. S.b. = brain derived from a naturally scrapie-sick sheep; h.b. = brain derived from a scrapie-free sheep control; h.k. = kidney derived from a scrapie-free sheep control, s.k. = kidney derived from a naturally scrapie-sick sheep. kD = kilo Dalton, M = medulla, C = cortex. (B) Detection of PrP^Sc by NaPTA Western blot in kidney homogenate derived from different Sarda sheep. Substantial individual variation of the Western blotting signal was observed among the sheep examined. Spike control = brain homogenate derived from a scrapie–sick sheep spiked into negative control kidney homogenate. h.b. = brain derived from a scrapie-free sheep control. 2000–4000 µg of renal homogenate were used as a starting material to perform NaPTA precipitation.