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. 2003 Sep 1;17(17):2123–2137. doi: 10.1101/gad.1117903

Figure 8.

Figure 8.

Mutations in the che-1 locus eliminate adoption of the ASE(L/R) fate (class I phenotype). (A) ASE(L/R) expression of gcy-7::gfp (otIs3), lim-6::gfp (otIs114), gcy-5::gfp (ntIs1), and flp-6::gfp (otIs125) in adult wild-type and adult che-1 (ot27) mutant animals. ASE-specific expression of all reporters is lost in che-1 mutants. Arrows in the “lim-6::gfp” panel denote the excretory gland cell that expresses lim-6::gfp and the ADF or AFD neuron class in the flp-6::gfp panel; note that gfp expression in neither of these cell types is affected in che-1 mutants. Asterisks denote gut autofluorescence. (B) Quantification of che-1 effects on ASE gene expression patterns. (C) Schematic CHE-1 protein structure (drawn to scale), denoting the position of mutant alleles retrieved from our screen. The deletion breakpoints in ot66 have not been sequenced but are inferred by PCR.