Fig. 1.

Transpososomes and transposition reaction steps. Blue-green double lines represent transposon end DNA, and pink double lines represent transposon-flanking DNA. Red double lines are the new target DNA. Rectangles indicate the transposase binding sequences. Transposase molecules are shown as green ovals. Small arrows indicate the nucleophilic attack either by water molecules or by 3′-OH with the arrowheads pointing to the scissile phosphate. Next to the STC, the DNA substrates used for the disintegration reaction are depicted.