Fig. 2.
Differential binding of netrin-G1 and -G2 to the NGL family proteins. Recombinant myc-tagged proteins of mouse netrin-G1 and -G2 were added to the HEK293T cells expressing NGLs. Surface binding of ligands was immunocytochemically detected with anti-myc antibody. Netrin-G1 specifically bound to NGL-1 (A), but not NGL-2-expressing cells (B). In contrast, netrin-G2 bound to NGL-2-expressing cells (D) without affinity to NGL-1 (C) (Scale bar: 20 μm). Binding of serially diluted netrin-G1 (E) and netrin-G2 (F) proteins to the sensorchip derivatized with NGL proteins was monitored by BIAcore biosensor, and affinities (Kd) of the interaction were determined.