Figure 4.

Fob1 is associated primarily with the NTS1 region. (A) Representative graph showing the association of Fob1–TAP across an rDNA repeat. Most of the protein is concentrated within NTS1. Two smaller peaks are observed at the 5S and near the start of the 35S rRNA genes. (B) Examples of ChIP data showing the association of Fob1–TAP with the 25S of rDNA. CUP1 is not significantly enriched in Fob1–TAP immunoprecipitations. (C) ChIP experiments showing the association of Fob1–TAP with the RFB region of NTS1. Labels are as in Figure 1. (F) Fob1–TAP cells. Primer reference numbers below the panels correspond to PCR products in A.