Figure 5.

Fob1 is required for the association of Net1 and Sir2 with NTS1. (A) Graph showing the association of Net1–TAP and Sir2–TAP with rDNA in fob1Δ cells. Net1 and Sir2 associate with the Pol I transcription initiation region and part of the 35S coding region. (B) Examples of ChIP experiments in fob1Δ cells showing reduced binding of both Net1–TAP and Sir2–TAP to the RFB region of NTS1. Panels show examples from both FOB1⁺ (upper set) and fob1Δ cells (lower set). CUP1 primers were used as a negative control. (C) Net1–TAP and Sir2–TAP associate with the NTS2/18S in both FOB1⁺ (upper set) and fob1Δ cells (lower set). Labels are as described in Figure 1, and locations of PCR products are shown below the graph in A.