Table 4.
Steady-state kinetic parameters for nucleotide incorporation opposite an abasic site by Polκ
| DNA substrate | Sequence | dNTP | kcat (min-1) | Km (μM) | kcat/Km (μM-1 min-1) |
|---|---|---|---|---|---|
| 11 | GCA | dATP | 1.6 ± 0.057 | 235 ± 27 | 0.007 |
| CGTXAT | dTTP | 0.090 ± 0.0056 | 212 ± 55 | 0.0004 | |
| dCTP | 0.19 ± 0.024 | 409 ± 180 | 0.0005 | ||
| dGTP | 0.10 ± 0.0068 | 281 ± 62 | 0.0004 | ||
| 12 | GCA | dATP | 0.25 ± 0.017 | 269 ± 47 | 0.0009 |
| CGTXGT | dTTP | n.d. | n.d. | <0.0001 | |
| dCTP | n.d. | n.d. | <0.0001 | ||
| dGTP | 0.074 ± 0.005 | 321 ± 88 | 0.0002 | ||
| 13 | GCA | dATP | 0.44 ± 0.013 | 216 ± 17 | 0.002 |
| CGTXCT | dTTP | n.d. | n.d. | <0.0001 | |
| dCTP | 0.069 ± 0.0064 | 295 ± 96 | 0.0002 | ||
| dGTP | 0.046 ± 0.0040 | 296 ± 82 | 0.0002 |
(n.d.) No nucleotide incorporation was detected; (X) abasic site.