Figure 2.

Schematic of domain structure of the EPA1 homologs. The sequences of EPA1-5 have been deposited with GenBank (accession nos. AY344225 and AY344226). The N-terminal domains of EPA1, EPA2, EPA3, EPA4, and EPA5 are indicated in yellow. The exact breakpoint between the N-terminal and C-terminal domains is somewhat arbitrary, but in general is marked by the first sequence that is highly enriched for Ser or Thr residues. The homology between the N-terminal ligand binding domains is detailed in Table 1. The C-terminal domains are characterized by several repeated sequences. For details and full amino acid sequence of the longer repeats, see the GenBank files indicated above. The green boxes in Epa1p indicate a 40-amino acid repeat (VRS.. .SKM) specific for that protein. The red boxes in Epa2p, Epa4p, and Epa5p indicate stretches almost entirely made up of Ser residues. The orange boxes in Epa2p and Epa3p indicate regions composed of three different repeats (HNPSS, VNPSS, and KPADPSPADPS), themselves repeated multiple times. The blue boxes in Epa2p-Epa5p indicate a 56-amino acid motif (DSFSL.. .NNGDS) found in all four proteins; the black stripes in these same genes indicate a 14-amino acid repeat (PSPNGGGSGNNGNT).