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Expression analysis of EPA1, EPA2, EPA3, and EPA4/5 by RAP1 and SIR3. Wild-type and the mutant strains, rap1-21 and sir3Δ, were assayed for EPA1, EPA2, EPA3, and EPA4/5 expression by RT-PCR (see Materials and Methods). ACT1 was used as the control. (-RT) Controls without RT; (G) genomic control.
