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. 2003 Sep 15;17(18):2245–2258. doi: 10.1101/gad.1121003

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Copyright © 2003, Cold Spring Harbor Laboratory Press

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Figure 5. — Silencing effect on the URA3 gene located in the intergenic regions at the EPA1 and EPA4/5 clusters. (Top) Strains were constructed carrying Tn7 insertions in the intergenic regions of ISC1 and HYR1 (A), EPA1 and EPA2 (B), EPA2 and EPA3 (C), and EPA3 and telomere (D). (Bottom) Strains were constructed carrying Tn7 insertions in the intergenic regions of PET56 and EPA5 (E), EPA5 and EPA4 (F), and EPA4 and telomere (G). Cells were inoculated at the same O.D.₆₀₀ nm and grown in YPD for 13 doublings, and 10-fold serial dilutions were spotted on YPD, SC-Ura, and SC + 5FOA (see Materials and Methods). Cells that are Ura⁺ die on SC + 5FOA plates. Thus only cells in which the URA3 gene is transcriptionally repressed can grow on this medium. Triangles represent Tn7 insertions, arrows indicate direction of transcription (T, telomere), and numbers in kilobases are the distance from telomere to the insertion point.

Figure 5. — Silencing effect on the URA3 gene located in the intergenic regions at the EPA1 and EPA4/5 clusters. (Top) Strains were constructed carrying Tn7 insertions in the intergenic regions of ISC1 and HYR1 (A), EPA1 and EPA2 (B), EPA2 and EPA3 (C), and EPA3 and telomere (D). (Bottom) Strains were constructed carrying Tn7 insertions in the intergenic regions of PET56 and EPA5 (E), EPA5 and EPA4 (F), and EPA4 and telomere (G). Cells were inoculated at the same O.D.₆₀₀ nm and grown in YPD for 13 doublings, and 10-fold serial dilutions were spotted on YPD, SC-Ura, and SC + 5FOA (see Materials and Methods). Cells that are Ura⁺ die on SC + 5FOA plates. Thus only cells in which the URA3 gene is transcriptionally repressed can grow on this medium. Triangles represent Tn7 insertions, arrows indicate direction of transcription (T, telomere), and numbers in kilobases are the distance from telomere to the insertion point.

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