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Silencing of the URA3 gene located in the intergenic regions at the EPA clusters depends on SIR3 and RAP1. The Tn7 insertions from Figure 5 and this figure were introduced in the sir3Δ and rap1-21 strains, and their ability to grow on SC + 5FOA plates was scored. (Top) Insertions at the EPA1 locus. (Bottom) Insertions at the EPA4/5 locus. (See Fig. 5 legend).
