Table 2.

Virulence of C. glabrata (hyrl epa1 epa2 epa3)Δ

	Kidney								Liver			Spleen
	Exp. 1			Exp 2		Exp. 3			Exp. 1			Exp. 1
Strain	Wild	M465	M475	Wild	M475	Wild	M465	M475	Wild	M465	M475	Wild	M465	M475
Sample size	10	10	10	10	10	10	8	9	10	10	10	10	10	10
Min. (×10−2)	2500	100	790	1900	500	6400	200	2700	900	100	520	10,600	270	18,000
Max. (×10−2)	122,000	15,300	45,000	49,000	48,000	57,200	31,700	37,100	6500	3700	5400	42,000	61,000	82,000
Mean (×10−2)	31,430	4520	14,219	14,100	6830	22,520	9980	13,140	2570	2200	2705	24,390	32,207	39,300
P-valuea	—	0.008	0.04	—	0.008	—	0.05	0.12	—	0.91	1	—	0.16	0.06

Balb/C mice were infected by tail vein injection with 2 × 10⁷ cells per strain and 10 mice were used per experiment per strain. Mice were sacrificed at day 7 after infection and kidney, liver, and spleen were recovered. The organs were homogenized and dilutions were plated on YPD Pen/Str plates, and CFU were scored (see Materials and Methods). Strains BG462 (wt), BG465 (mutant 1), and BG475 (mutant 2) were used in these experiments (see Supplemental Material, Table S1). There is a significant three- to five-fold reduction in kidney colonization, but no difference in colonization of spleen or liver. Representative counts are shown for spleen and liver from the first experiment; in the subsequent experiments as well there was no significant difference in recovered CFUs for the three strains for spleen and liver (data not shown).

^aKruskal-Wallis test.