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. 2003 Sep 15;17(18):2298–2307. doi: 10.1101/gad.1111603

Figure 3.

Figure 3.

Hoxa7 and Hoxa9 are independently required for efficient in vitro immortalization by MLL-ENL. (A) Representative results (CFUs/10,000 plated cells) of serial myeloid replating assays are shown for myeloid progenitors transduced with vector alone (V), MLL-ENL (ME), or E2A-HLF (EH) as indicated. Genotypes of BM cells used for transductions are indicated above the panels. (B) Morphologies for colony types observed in methylcellulose assays initiated with cells transduced by MLL-ENL (rows 1 and 2) or E2A-HLF (row 3). Genotypes of transduced cells are indicated above the panels. (C) Coexpression of Hoxa9 in combination with MLL-ENL rescues in vitro immortalization of Hoxa9-/- BM cells. Results are shown as CFU/10,000 plated cells. Transduced constructs are indicated below, along with indications of ability to establish IL-3-dependent cell lines in liquid media.