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. Author manuscript; available in PMC: 2007 Sep 4.
Published in final edited form as: Prog Brain Res. 2007;163C:399–816. doi: 10.1016/S0079-6123(07)63023-4

Figure 1.

Figure 1

Effects of ovariectomy and estrogen replacement on the number of hippocampal spine synapses. Young adult female Sprague-Dawley rats (250 g) were ovariectomized and one week later, they received either 10 μg/rat/day estradiol-benzoate (EB, solid columns) or 200 μl/rat/day sesame oil vehicle (Oil, open columns) subcutaneously for two days. Two days after the last injection, the animals were sacrificed by transcardial perfusion of fixative, and their brains were processed for electron microscopic stereological analysis. Spine synapses were counted in the CA1 and CA3 strata radiata, and in the molecular layer of the dentate gyrus (DG). *Significantly different from the corresponding Oil group (t-test, p<0.001 in CA1 and DG, p<0.01 in CA3).